In recent years, the field of DNA nanotechnology has developed a number of strategies for the construction of nanometer-scale structures by exploiting the natural properties of DNA. One such strategy is DNA origami, in which a long single-stranded DNA template strand is ‘folded’ into a particular shape by hybridising with many shorter staple strands (1). These staples crosslink the template strand at known locations, folding the template into predictable shapes. The resulting nanostructures can be used for a wide variety of applications, including the precise positioning, or scaffolding, of individual molecules such as proteins, nanoparticles or quantum dots. With the right scaffold, it may even be possible to initiate the self-assembly of large protein complexes in vitro, providing the basis for a host of experiments and potentially leading to major advances in structural biology and bio-inspired technologies. In order to do this, we may need to mimic natural scaffolds that occur in vivo, and in the case of ring-like complexes such as the bacterial flagellar motor, this means circular, ring-like scaffolds. DNA origami nanotubes with rotational symmetry could provide identical attachment points in circular arrays, and hence could be used for scaffolding these complexes. Here I present a method which facilitates the construction of such nanotubes, which provide a customisable number of identical attachment points in a circular array. The method also allows for the design of star-shaped ‘double-walled’ nanotubes, which provide additional rigidity and customisability. These double-walled nanotubes can be further rigidified by the incorporation of the antiparallel-parallel-antiparallel (APA) staple, a novel DNA origami crosslink that connects sections of the template strand in a rigid three-helix bundle, providing a new building block for DNA origami.