The nucleosome remodelling and deacetylase (NuRD) complex is essential for the development of complex animals. NuRD has roles in regulating gene expression and repairing damaged DNA. The complex comprises at least six proteins with two or more paralogues of each protein routinely identified when the complex is purified from cell extracts. To understand the structure and function of NuRD, a map of direct subunit interactions is needed. Dozens of published studies have attempted to define sets of direct inter-subunit connectivity. With this work we propose that conclusions reported in many such studies are in fact ambiguous. Combining existing information on NuRD structure with a protocol designed to minimize false positives, we present a conservative and robust interaction map for the NuRD complex. As a result, we also suggest a 3D model of the complex. The issues and strategies shown in the poster presentation are applicable to the analysis of a wide range of multi-subunit complexes.